cutsmart buffer Search Results


cutsmart buffer 10x  (New England Biolabs)
99
New England Biolabs cutsmart buffer 10x
Cutsmart Buffer 10x, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cutsmart buffer 10x - by Bioz Stars, 2026-04
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cutsmart buffer  (Nacalai)
90
Nacalai cutsmart buffer
Cutsmart Buffer, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cutsmart buffer - by Bioz Stars, 2026-04
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1x cutsmart buffer  (Fisher Scientific)
90
Fisher Scientific 1x cutsmart buffer
1x Cutsmart Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
1x cutsmart buffer - by Bioz Stars, 2026-04
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cutsmart buffer  (Signal Recovery)
90
Signal Recovery cutsmart buffer
a Fluorescence signal kinetics of AsCas12-mediated detection for SARS-CoV-2 S gene synthetic DNA (10 10 copies) with indicated chemical additives in the reaction mix. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. b Effects of L-proline on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using different types of Cas protein representing differential states of denaturing in batch #1 <t>CutSmart</t> reaction buffer. L-proline is added in the CRISPR detection mix. Three types <t>of</t> <t>AsCas12a</t> protein are used in this assay - type 1: fresh protein from frozen stock; type 2: protein left at room temperature for 48 hours; and type 3: protein undergone multiple freeze-thaw cycles during 48 hours. Endpoint (60 min) recording of fluorescence detection signals are shown. w/o template means no input in CRISPR detection system; differential amount of L-proline are added in the reaction mix: no L-proline (-), with indicated amount of L-proline (+). a.u., arbitrary unit. c Batch effect of reaction buffers on AsCas12a-based detection and L-proline’s enhancement. The same samples used in b are gone through the similar assays only except that the reaction buffer changes to batch #2. a.u., arbitrary unit. d Effects of L-proline on AsCas12a-based detection for SARS-CoV-2 S gene DNA template with heat-denatured AsCas12a proteins. AsCas12a protein is pre-heated for 2, 4 and 6 hours in 42°C, and then is examined for their capability on CRISPR detection in the absence (-) or presence (+) of 0.5 M L-proline in the detection mix. Fluorescence signal kinetics is shown. a.u., arbitrary unit. e Effects of BSA and L-proline addition on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using Takara-T and Takara-K buffers. Endpoint (60 min) recording of fluorescence detection signals is shown. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. f Comparison of different sources of BSA on AsCas12-mediated SARS-CoV-2 N gene DNA template detection in Takara-K buffer. Different vendors of BSA are used. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit. g, h Evaluation of multiple batches (#3 - #7) of CutSmart buffer for the effect on AsCas12a (g) and LwaCas13a (h) detection capability without (-) or with (+) 0.5 M L-proline addition using SARS-CoV-2 N gene DNA (g) or RNA (h) templates. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit.
Cutsmart Buffer, supplied by Signal Recovery, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cutsmart buffer/product/Signal Recovery
Average 90 stars, based on 1 article reviews
cutsmart buffer - by Bioz Stars, 2026-04
90/100 stars
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cutsmart buffer #2  (Signal Recovery)
90
Signal Recovery cutsmart buffer #2
a Fluorescence signal kinetics of AsCas12-mediated detection for SARS-CoV-2 S gene synthetic DNA (10 10 copies) with indicated chemical additives in the reaction mix. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. b Effects of L-proline on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using different types of Cas protein representing differential states of denaturing in batch #1 <t>CutSmart</t> reaction buffer. L-proline is added in the CRISPR detection mix. Three types <t>of</t> <t>AsCas12a</t> protein are used in this assay - type 1: fresh protein from frozen stock; type 2: protein left at room temperature for 48 hours; and type 3: protein undergone multiple freeze-thaw cycles during 48 hours. Endpoint (60 min) recording of fluorescence detection signals are shown. w/o template means no input in CRISPR detection system; differential amount of L-proline are added in the reaction mix: no L-proline (-), with indicated amount of L-proline (+). a.u., arbitrary unit. c Batch effect of reaction buffers on AsCas12a-based detection and L-proline’s enhancement. The same samples used in b are gone through the similar assays only except that the reaction buffer changes to batch #2. a.u., arbitrary unit. d Effects of L-proline on AsCas12a-based detection for SARS-CoV-2 S gene DNA template with heat-denatured AsCas12a proteins. AsCas12a protein is pre-heated for 2, 4 and 6 hours in 42°C, and then is examined for their capability on CRISPR detection in the absence (-) or presence (+) of 0.5 M L-proline in the detection mix. Fluorescence signal kinetics is shown. a.u., arbitrary unit. e Effects of BSA and L-proline addition on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using Takara-T and Takara-K buffers. Endpoint (60 min) recording of fluorescence detection signals is shown. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. f Comparison of different sources of BSA on AsCas12-mediated SARS-CoV-2 N gene DNA template detection in Takara-K buffer. Different vendors of BSA are used. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit. g, h Evaluation of multiple batches (#3 - #7) of CutSmart buffer for the effect on AsCas12a (g) and LwaCas13a (h) detection capability without (-) or with (+) 0.5 M L-proline addition using SARS-CoV-2 N gene DNA (g) or RNA (h) templates. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit.
Cutsmart Buffer #2, supplied by Signal Recovery, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cutsmart buffer #2/product/Signal Recovery
Average 90 stars, based on 1 article reviews
cutsmart buffer #2 - by Bioz Stars, 2026-04
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cutsmart buffer  (BioNordika Oy)
90
BioNordika Oy cutsmart buffer
a Fluorescence signal kinetics of AsCas12-mediated detection for SARS-CoV-2 S gene synthetic DNA (10 10 copies) with indicated chemical additives in the reaction mix. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. b Effects of L-proline on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using different types of Cas protein representing differential states of denaturing in batch #1 <t>CutSmart</t> reaction buffer. L-proline is added in the CRISPR detection mix. Three types <t>of</t> <t>AsCas12a</t> protein are used in this assay - type 1: fresh protein from frozen stock; type 2: protein left at room temperature for 48 hours; and type 3: protein undergone multiple freeze-thaw cycles during 48 hours. Endpoint (60 min) recording of fluorescence detection signals are shown. w/o template means no input in CRISPR detection system; differential amount of L-proline are added in the reaction mix: no L-proline (-), with indicated amount of L-proline (+). a.u., arbitrary unit. c Batch effect of reaction buffers on AsCas12a-based detection and L-proline’s enhancement. The same samples used in b are gone through the similar assays only except that the reaction buffer changes to batch #2. a.u., arbitrary unit. d Effects of L-proline on AsCas12a-based detection for SARS-CoV-2 S gene DNA template with heat-denatured AsCas12a proteins. AsCas12a protein is pre-heated for 2, 4 and 6 hours in 42°C, and then is examined for their capability on CRISPR detection in the absence (-) or presence (+) of 0.5 M L-proline in the detection mix. Fluorescence signal kinetics is shown. a.u., arbitrary unit. e Effects of BSA and L-proline addition on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using Takara-T and Takara-K buffers. Endpoint (60 min) recording of fluorescence detection signals is shown. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. f Comparison of different sources of BSA on AsCas12-mediated SARS-CoV-2 N gene DNA template detection in Takara-K buffer. Different vendors of BSA are used. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit. g, h Evaluation of multiple batches (#3 - #7) of CutSmart buffer for the effect on AsCas12a (g) and LwaCas13a (h) detection capability without (-) or with (+) 0.5 M L-proline addition using SARS-CoV-2 N gene DNA (g) or RNA (h) templates. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit.
Cutsmart Buffer, supplied by BioNordika Oy, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cutsmart buffer/product/BioNordika Oy
Average 90 stars, based on 1 article reviews
cutsmart buffer - by Bioz Stars, 2026-04
90/100 stars
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cutsmart buffer  (Sangon Biotech)
90
Sangon Biotech cutsmart buffer
a Fluorescence signal kinetics of AsCas12-mediated detection for SARS-CoV-2 S gene synthetic DNA (10 10 copies) with indicated chemical additives in the reaction mix. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. b Effects of L-proline on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using different types of Cas protein representing differential states of denaturing in batch #1 <t>CutSmart</t> reaction buffer. L-proline is added in the CRISPR detection mix. Three types <t>of</t> <t>AsCas12a</t> protein are used in this assay - type 1: fresh protein from frozen stock; type 2: protein left at room temperature for 48 hours; and type 3: protein undergone multiple freeze-thaw cycles during 48 hours. Endpoint (60 min) recording of fluorescence detection signals are shown. w/o template means no input in CRISPR detection system; differential amount of L-proline are added in the reaction mix: no L-proline (-), with indicated amount of L-proline (+). a.u., arbitrary unit. c Batch effect of reaction buffers on AsCas12a-based detection and L-proline’s enhancement. The same samples used in b are gone through the similar assays only except that the reaction buffer changes to batch #2. a.u., arbitrary unit. d Effects of L-proline on AsCas12a-based detection for SARS-CoV-2 S gene DNA template with heat-denatured AsCas12a proteins. AsCas12a protein is pre-heated for 2, 4 and 6 hours in 42°C, and then is examined for their capability on CRISPR detection in the absence (-) or presence (+) of 0.5 M L-proline in the detection mix. Fluorescence signal kinetics is shown. a.u., arbitrary unit. e Effects of BSA and L-proline addition on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using Takara-T and Takara-K buffers. Endpoint (60 min) recording of fluorescence detection signals is shown. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. f Comparison of different sources of BSA on AsCas12-mediated SARS-CoV-2 N gene DNA template detection in Takara-K buffer. Different vendors of BSA are used. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit. g, h Evaluation of multiple batches (#3 - #7) of CutSmart buffer for the effect on AsCas12a (g) and LwaCas13a (h) detection capability without (-) or with (+) 0.5 M L-proline addition using SARS-CoV-2 N gene DNA (g) or RNA (h) templates. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit.
Cutsmart Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cutsmart buffer/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
cutsmart buffer - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


a Fluorescence signal kinetics of AsCas12-mediated detection for SARS-CoV-2 S gene synthetic DNA (10 10 copies) with indicated chemical additives in the reaction mix. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. b Effects of L-proline on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using different types of Cas protein representing differential states of denaturing in batch #1 CutSmart reaction buffer. L-proline is added in the CRISPR detection mix. Three types of AsCas12a protein are used in this assay - type 1: fresh protein from frozen stock; type 2: protein left at room temperature for 48 hours; and type 3: protein undergone multiple freeze-thaw cycles during 48 hours. Endpoint (60 min) recording of fluorescence detection signals are shown. w/o template means no input in CRISPR detection system; differential amount of L-proline are added in the reaction mix: no L-proline (-), with indicated amount of L-proline (+). a.u., arbitrary unit. c Batch effect of reaction buffers on AsCas12a-based detection and L-proline’s enhancement. The same samples used in b are gone through the similar assays only except that the reaction buffer changes to batch #2. a.u., arbitrary unit. d Effects of L-proline on AsCas12a-based detection for SARS-CoV-2 S gene DNA template with heat-denatured AsCas12a proteins. AsCas12a protein is pre-heated for 2, 4 and 6 hours in 42°C, and then is examined for their capability on CRISPR detection in the absence (-) or presence (+) of 0.5 M L-proline in the detection mix. Fluorescence signal kinetics is shown. a.u., arbitrary unit. e Effects of BSA and L-proline addition on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using Takara-T and Takara-K buffers. Endpoint (60 min) recording of fluorescence detection signals is shown. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. f Comparison of different sources of BSA on AsCas12-mediated SARS-CoV-2 N gene DNA template detection in Takara-K buffer. Different vendors of BSA are used. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit. g, h Evaluation of multiple batches (#3 - #7) of CutSmart buffer for the effect on AsCas12a (g) and LwaCas13a (h) detection capability without (-) or with (+) 0.5 M L-proline addition using SARS-CoV-2 N gene DNA (g) or RNA (h) templates. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit.

Journal: bioRxiv

Article Title: A Chemical-Enhanced System for CRISPR-Based Nucleic Acid Detection

doi: 10.1101/2021.03.28.437376

Figure Lengend Snippet: a Fluorescence signal kinetics of AsCas12-mediated detection for SARS-CoV-2 S gene synthetic DNA (10 10 copies) with indicated chemical additives in the reaction mix. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. b Effects of L-proline on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using different types of Cas protein representing differential states of denaturing in batch #1 CutSmart reaction buffer. L-proline is added in the CRISPR detection mix. Three types of AsCas12a protein are used in this assay - type 1: fresh protein from frozen stock; type 2: protein left at room temperature for 48 hours; and type 3: protein undergone multiple freeze-thaw cycles during 48 hours. Endpoint (60 min) recording of fluorescence detection signals are shown. w/o template means no input in CRISPR detection system; differential amount of L-proline are added in the reaction mix: no L-proline (-), with indicated amount of L-proline (+). a.u., arbitrary unit. c Batch effect of reaction buffers on AsCas12a-based detection and L-proline’s enhancement. The same samples used in b are gone through the similar assays only except that the reaction buffer changes to batch #2. a.u., arbitrary unit. d Effects of L-proline on AsCas12a-based detection for SARS-CoV-2 S gene DNA template with heat-denatured AsCas12a proteins. AsCas12a protein is pre-heated for 2, 4 and 6 hours in 42°C, and then is examined for their capability on CRISPR detection in the absence (-) or presence (+) of 0.5 M L-proline in the detection mix. Fluorescence signal kinetics is shown. a.u., arbitrary unit. e Effects of BSA and L-proline addition on AsCas12-mediated SARS-CoV-2 N gene DNA template detection using Takara-T and Takara-K buffers. Endpoint (60 min) recording of fluorescence detection signals is shown. w/o template means no input in CRISPR detection system; differential amount of chemicals are added in the reaction mix: no chemical (-), with indicated amount of chemical (+). a.u., arbitrary unit. f Comparison of different sources of BSA on AsCas12-mediated SARS-CoV-2 N gene DNA template detection in Takara-K buffer. Different vendors of BSA are used. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit. g, h Evaluation of multiple batches (#3 - #7) of CutSmart buffer for the effect on AsCas12a (g) and LwaCas13a (h) detection capability without (-) or with (+) 0.5 M L-proline addition using SARS-CoV-2 N gene DNA (g) or RNA (h) templates. Endpoint (60 min) recording of fluorescence detection signals are shown. a.u., arbitrary unit.

Article Snippet: These batch deviated results suggest that certain ingredients within CutSmart buffer #2 may retain the activity of AsCas12a even if after mild deterioration treatment, and L-proline’s effect on AsCas12a is probably masked by such endogenous signal recovery.

Techniques: Fluorescence, CRISPR, Comparison